TY  - JOUR
T1  - A Novel Pig Gene, PFKFB1, Differentially Expressed in the Muscle Tissues from Wujin Pigs and Large White Pigs
AU - Yonggang, Liu 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 4
SP  - 421
EP  - 427
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.421.427
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.421.427
KW  - gene
KW  -Pig
KW  -mRNA
KW  -differential display
KW  -muscle tissue
KW  -PFKFB1
KW  -China
AB  - The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large white pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the Rapid Amplification of Cdna Ends (RACE) method. The open reading frame of this gene encodes a protein of 428 amino acids which is homologous with the 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 1 (PFKFB1) of 6 species: cattle (97%), human (97%), horse (96%), rhesus monkey (96%), rat (94%) and mouse (94%). This newly identified gene was respectively defined as the swine PFKFB1 gene and had been assigned GeneID: 100233197. The phylogenetic tree analysis revealed that the swine PFKFB1 gene has a closer genetic relationship with the PFKFB1 gene of cattle. The tissue expression analysis indicated that the swine PFKFB1 gene has a broad tissue distribution. The experiment is the first to establish the primary foundation for further research on the swine PFKFB1 gene.
ER  -