TY  - JOUR
T1  - Simultaneous Detection and Virulence Characterisation of <I>Dichelobacter nodosus</I> from Ovine Footrot by Multiplex PCR
AU - Taku, A.K. AU - Beigh, M.M. AU - Kumar, B. AU - Kumar, S. AU - Bhat, M.A. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 24
SP  - 3192
EP  - 3195
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.3192.3195
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.3192.3195
KW  - Footrot
KW  -Gelatin gel test
KW  -intA gene
KW  -multiplex PCR
KW  -16S rRNA
KW  -India
AB  - Out of 262 swab samples from feet of clinically footrot affected sheep from Jammu and Kashmir, 135 (52.0%) detected positive for <I>Dichelobacter nodosus</I> by <I>16S rRNA</I> gene specific PCR. Out of these135 positive samples, 82 (61.74%) tested positive for <I>intA</I> gene carried by virulent strains. A mulplex PCR for simultaneous detection and virulence characterization of <I>D. nodosus</I> in clinical samples was therefore devised. The test detected 77 (57.03%) samples positive for virulent <I>D. nodosus</I>. The results were comparable and the success of the multiplex PCR was established. Out of 30 randomly selected isolates subjected to gelatin gel test, 24 isolates with <I>intA </I>gene produced thermostable protease while six isolates without <I>intA</I> gene revealed the production of thermolabile protease. This indicated a good co-relation between presence of <I>intA</I> gene and gelatine gel test in determination of the<I> D. nodosus</I> virulence.
ER  -