TY  - JOUR
T1  - <I>S. enteritidis</I> Genome Loads in the Tissues of Reproductive Organs in Laying Duck
AU - He, Guang-Zhi AU - Deng, Shu-Xuan 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 23
SP  - 3165
EP  - 3168
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.3165.3168
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.3165.3168
KW  - Salmonella enteritidis
KW  -reproductive organs
KW  -duck
KW  -PCR
KW  -China
AB  - The objective of this study was to determine the replication kinetics of <I>Salmonella enteritidis</I> genome loads (<I>S. enteritidis</I>) in reproductive organs of laying duck after oral challenge. We conducted serovar-specific real-time PCR for <I>S. enteritidis</I> to detect the genomic DNA of <I>S. enteritidis</I> in laying duck at different time points. To validate these results, the Indirect Fluorescent Antibody (IFA) technique was employed too. The results showed that <I>S. enteritidis</I> was consistently detected in all the samples. However, the number of bacteria started decreasing by 3-4 days and by 6 days, the concentration of <I>S. enteritidis</I> DNA was below the detection limits of the PCR assay except the spleen and vagina. In conclusion, this study will help in understanding the pathogenesis of <I>S. enteritidis</I> infection <I>in vivo</I>.
ER  -