TY  - JOUR
T1  - Enrichment and Short Term Culture of the Ovine Gonocyte
AU - Borjigin, Uyunbilig AU - Zhou, Xin AU - Han, Xuejie AU - Li, Rongfeng AU - Herrid, Muren AU - Bou, Shorgan 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 22
SP  - 2936
EP  - 2942
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2936.2942
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2936.2942
KW  - Ovine
KW  -gonocyte
KW  -cell culture
KW  -immunostaining
KW  -spermatogonia
KW  -Australia
AB  - The aim of this study was to investigate the effects of two types of ovine testis cells population as feeder cell on <I>in vitro</I> culture of the enriched ovine gonocytes. The feeder cell populations were prepared from 5-6 months old ovine testis. The 1-2 months old neonatal rams were used to isolate germ cells through a two step enzymatic digestion followed by differential plating for SSCs enrichment. Isolated and enriched cells were characterized by using PLZF and VASA antibody. During the 1st week of culture, gonocyte formed pairs and chains of type A spermatogonia. After 1 week, colonies started to increase in size. About 2 weeks later, more colonies in type II feeder group kept undifferentiated and looks more effective regarding colony formation of spermatogonia compared with type I feeder group in the ovine spermatogonial stem cell culture system.
ER  -