TY  - JOUR
T1  - Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>ARL2</I>, <I>ARL3</I> and <I>ARL4C</I>
AU - Caode, Jiang AU - Yonggang, Liu AU - Xueshan, Xia 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 21
SP  - 2772
EP  - 2778
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2772.2778
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2772.2778
KW  - Sheep
KW  -ARL2
KW  -ARL3
KW  -ARL4C
KW  -tissue expression
KW  -China
AB  - The complete coding sequences of three sheep genes-<I>ARL2</I>, <I>ARL3</I> and <I>ARL4C</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Sequence analysis revealed that sheep <I>ARL2</I> gene encodes a protein of 184 amino acids that shares high homology with the ADP-Ribosylation factor-like 2 (ARL2) proteins of 9 species-cattle (100%), pig (99%), human (99%), dog (98%), mouse (96%), rat (95%), western clawed frog (90%), African clawed frog (89%) and channel catfish (85%). The sheep <I>ARL3</I> gene encodes a protein of 182 amino acids that shares high homology with the ADP-Ribosylation factor-like 3 (ARL3) proteins of 9 species-cattle (100%), pig (99%), horse (98%), human (98%), mouse (98%), rat (97%), African clawed frog (97%), red jungle fowl (96%) and zebrafish (94%). The sheep <I>ARL4C</I> gene encodes a protein of 192 amino acids that shares high homology with the ADP-Ribosylation factor-like 4C (ARL4C) proteins of 11 species-cattle (100%), human (100%), chimpanzee (100%), mouse (100%), taeniopygia guttata (98%), gray short-tailed opossum (98%), green anole (98%), atlantic salmon (96%), rainbow smelt (96%), zebrafish (95%) and African clawed frog (91%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302310, 100302311 and 100302312. The phylogenetic analysis revealed that the sheep <I>ARL2</I> and <I>ARL3</I> genes both have closer genetic relationships with the <I>ARL2</I> and <I>ARL3</I> genes of cattle. The sheep <I>ARL4C</I> gene has a closer genetic relationship with the <I>ARL4C</I> genes of cattle, human, chimpanzee and mouse. Tissue expression profile analysis was also carried out and results demonstrated that sheep <I>ARL2</I>, <I>ARL3</I> and <I>ARL4C</I> genes were all generally but differentially expressed in detected tissues.
ER  -