TY  - JOUR
T1  - Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (<I>Ovis aries</I>) Genes <I>SLC39A1</I>, <I>SLC39A2</I> and <I>SLC39A7</I>
AU - Xueshan, Xia AU - Yonggang, Liu AU - Caode, Jiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 20
SP  - 2699
EP  - 2705
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2699.2705
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2699.2705
KW  - China
KW  -genetic relatiuonship
KW  -Sheep
KW  -SLC39A1
KW  -SLC39A2
KW  -SLC39A7
KW  -tissue expression
AB  - The complete coding sequences of three sheep genes <I>SLC39A1</I>, <I>SLC39A2</I> and <I>SLC39A7</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Sequence analysis revealed that sheep <I>SLC39A1</I> gene encodes a protein of 324 amino acids that shares high homology with the solute carrier family 39 (zinc transporter), member1 (SLC39A1) proteins of eleven species-goat (99%), cattle (99%), pig (95%), horse (94%), rhesus monkey (94%), chimpanzee (94%), human (94%), rabbit (94%), crab-eating macaque (93%), mouse (93%) and rat (92%). The sheep <I>SLC39A2</I> gene encodes a protein of 309 amino acids that shares high homology with the solute carrier family 39 (zinc transporter), member 2 (SLC39A2) proteins of eleven species-goat (98%), cattle (95%), horse (81%), giant panda (80%), human (79%), rhesus monkey (78%), chimpanzee (78%), rabbit (77%), Northern white-cheeked gibbon (77%), mouse (75%) and rat (74%). The sheep <I>SLC39A7</I> gene encodes a protein of 469 amino acids that shares high homology with the solute carrier family 39 (zinc transporter), member 7(SLC39A7) proteins of thirteen species-cattle (98%), dog (93%), pig (94%), chimpanzee (93%), human (93%), horse (93%), rat (93%), rhesus monkey (93%), white-tufted-ear marmoset (92%), Northern white-cheeked gibbon (92%), sumatran orangutan (92%), rabbit (89%) and mouse (86%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302552, 100302553 and 100302555. The phylogenetic analysis revealed that the sheep <I>SLC39A1</I> and <I>SLC39A2</I> genes both have closer genetic relationships with the <I>SLC39A1</I> and <I>SLC39A2</I> genes of goat. The sheep <I>SLC39A7</I> gene has a closer genetic relationship with the <I>SLC39A7</I> gene of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep <I>SLC39A1</I>, <I>SLC39A2</I> and <I>SLC39A7</I> genes were all generally but differentially expressed in detected tissues.
ER  -