TY  - JOUR
T1  - Molecular Cloning, Sequence Identification and Tissue Expression rofile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>RAB1A</I>, <I>RAB4A</I> and<I> RAB5A</I>
AU - Xueshan, Xia AU - Yonggang, Liu AU - Caode, Jiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 20
SP  - 2659
EP  - 2665
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2659.2665
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2659.2665
KW  - Sheep
KW  -RAB5A
KW  -RAB4A
KW  -RAB1A
KW  -tissue expression
KW  -China
AB  - The complete coding sequences of three sheep genes-<I>RAB1A</I>, <I>RAB4A</I> and <I>RAB5A</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Sequence analysis revealed that the sheep <I>RAB1</I>A gene encodes a protein of 204 amino acids that shares high homology with the RAB1A, member RAS oncogene family (RAB1A) proteins of nine species-human (98%), mouse (98%), rat (98%), pig (98%), zebrafish (98%), Western clawed frog (97%), Atlantic salmon (96%), disc abalone (92%) and great pond snail (87%). The sheep <I>RAB4A</I> gene encodes a protein of 218 amino acids that shares high homology with the RAB4A, member RAS oncogene family (RAB4A) proteins of eight species-cattle (100%), dog (99%), human (99%), rhesus monkey (99%), horse (99%), chicken (99%), rat (98%) and mouse (98%). The sheep <I>RAB5A</I> gene encodes a protein of 215 amino acids that shares high homology with the RAB5A, member RAS oncogene family (RAB5A) proteins of fifteen species-cattle (98%), rabbit (98%), dog (98%), horse (98%), pig (98%), sumatran orangutan (98%), human (97%), mouse (97%), rat (97%), chimpanzee (97%), chicken (95%), rhesus monkey (93%), African clawed frog (93%), Western clawed frog (93%) and zebrafish (92%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302065, 100302066 and 100302084. Phylogenetic analysis indicated that the sheep <I>RAB1A</I> gene has a closer genetic relationship with the <I>RAB1A</I> genes of human, mouse and rat. The sheep <I>RAB4A</I> and <I>RAB5A</I> genes both have closer genetic relationships with the <I>RAB4A</I> and <I>RAB5A </I>genes of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep <I>RAB1A</I>, <I>RAB4A</I> and <I>RAB5A</I> genes were all generally but differentially expressed in detected tissues.
ER  -