TY  - JOUR
T1  - Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>ARF3, ARF4</I> and <I>ARF5</I>
AU - Caode, Jiang AU - Yonggang, Liu 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 20
SP  - 2642
EP  - 2648
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2642.2648
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2642.2648
KW  - tissue expression
KW  -ARF5
KW  -ARF4
KW  -ARF3
KW  -Sheep
KW  -cattle
KW  -rat
AB  - The complete coding sequences of three sheep genes-<I>ARF3</I>, <I>ARF4</I> and <I>ARF5</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The nucleotide sequences of these three genes revealed that sheep <I>ARF3</I> gene encodes a protein of 181 amino acids that shares high homology with the ADP-Ribosylation Factor 3 (ARF3) proteins of five species human (99%), zebrafish (99%), African clawed frog (99%), pig (99%) and atlantic salmon (98%). The sheep <I>ARF4</I> gene encodes a protein of 180 amino acids that shares high homology with the ADP-Ribosylation Factor 4 (ARF4) proteins of third species-cattle (100%), platypus (99%), green anole (99%), pig (99%), rabbit (98%), African clawed frog (98%), red jungle fowl (98%), mouse (98%), zebrafish (96%), human (97%), atlantic salmon (92%), rainbow trout (92%) and <I>Caligus rogercresseyi</I> (89%). The sheep <I>ARF5</I> gene encodes a protein of 180 amino acids that shares high homology with the ADP-Ribosylation Factor 5 (ARF5) proteins of seven species human (100%), mouse (100%), rat (100%), gray short-tailed opossum (99%), chicken (98%), channel catfish (93%) and zebrafish (93%). Finally, these three novel sheep genes were assigned to GeneIDs; 100302303, 100302304 and 100302305. The phylogenetic analysis indicated that the sheep <I>ARF3</I> gene has closer genetic relationship with the <I>ARF3</I> gene of human. The sheep <I>ARF4</I> gene has closer genetic relationship with the <I>ARF4</I> gene of cattle and the sheep <I>ARF5</I> gene has closer genetic relationship with the <I>ARF5</I> genes of human, mouse and rat. Tissue expression profile analysis was also carried out and results demonstrated that sheep <I>ARF3</I>, <I>ARF4</I> and <I>ARF5</I> genes were differentially expressed in detected tissues.
ER  -