TY  - JOUR
T1  - Isolation, Sequence Identification and Tissue Expressio Profile of a Novel Sheep Gene-<I>SERPINF1</I>
AU - Caode, Jiang AU - Yonggang, Liu 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 19
SP  - 2593
EP  - 2598
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2593.2598
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2593.2598
KW  - Sheep
KW  -SERPINF1
KW  -RACE
KW  -tissue expression profile
KW  -sequence identification
KW  -China
AB  - The full-length cDNA sequences of one sheep gene, <I>SERPINF1</I> was amplified using the Rapid Amplification of cDNA Ends (RACE) method based on one pig EST sequence which was highly homologous to the coding sequence of human <I>SERPINF1</I> gene. Sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 416 amino acids that has high homology with the serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 (SERPINF1) of twelve species-bovine (96%), pig (91%), human (88%), chimpanzee (88%), horse (88%), crab-eating macaque (87%), dog (89%), domestic guinea pig (84%), mouse (85%), rat (83%), red jungle fowl (63%) and western clawed frog (55%) so that it can be defined as sheep <I>SERPINF1</I> gene. This novel sheep gene was assigned to GeneID: 100192425. The phylogenetic analysis revealed that the sheep <I>SERPINF1</I> gene has a closer genetic relationship with the <I>SERPINF1</I> gene of bovine. Tissue expression analysis indicated that the sheep <I>SERPINF1</I> gene is differentially expressed in detected tissues including spleen, muscle, skin, kidney, lung, liver, fat and heart. The experiment is the first to establish the primary foundation for further research on the sheep <I>SERPINF1</I> gene.
ER  -