TY  - JOUR
T1  - Porcine IFN-&#8226; is Biologically Active and Secreted in the Yeast <I>Pichia 
  pastoris</I> Expression System
AU - He, Dongsheng AU - Ning, Zhangyong AU - Yu, Xiaoli AU - Liao, Ming AU - Li, Kangning AU - Zhao, Yanzong 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 18
SP  - 2450
EP  - 2455
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2450.2455
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2450.2455
KW  - Porcine interferon-• (PoIFN-•)
KW  -Pichia pastoris
KW  -expression
KW  -activity
KW  -cells
KW  -biologically active
AB  - Porcine Interferon (PoIFN) is a multifunctional secretory 
  protein produced by animal immune cells. IFN signaling activates host cells 
  resulting in anti-viral and -tumor immune regulation in addition to other biological 
  functions. Here, the coding region of PoIFN-&#8226; was amplified from porcine 
  genomic DNA by Polymerase Chain Reaction (PCR) and was cloned into the yeast 
  <I>Pichia pastoris</I> expression system. This resulted in high levels of gene 
  expression. Furthermore, expression of PoIFN-&#8226; was successfully detected 
  by Western-blot. The relative molecular weight of the product was 25 and 27 
  kDa. The recombinant PoIFN-&#8226; exhibited cross-species antiviral activity 
  in Marc-145 and BHK-21 mammalian cell lines. In summary, the results demonstrate 
  that recombinant PoIFN-&#8226; cloned into <I>P. pastoris</I> is expressed, 
  secreted and biologically active in mammalian cells.
ER  -