TY  - JOUR
T1  - Development of PCR for the Identification of Porcine Circovirus Type 2 (PCV-2) Genotype PCV-2a and PCV-2b
AU - He, Dongsheng AU - Zhao, Yanzong AU - Su, Danping AU - Niu, Xiaoyun 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 18
SP  - 2398
EP  - 2401
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2398.2401
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2398.2401
KW  - porcine circovirus type 2b (PCV-2b)
KW  -porcine circovirus type 2a (PCV-2a)
KW  -Porcine circovirus type 2 (PCV-2)
KW  -agent
KW  -target
KW  -China
AB  - PCR was developed to evaluate for its ability to simultaneously detect viral infections of swine. Specific primers were designed for each sub-type of porcine circovirus type 2 (PCV-2); porcine circovirus type 2a (PCV-2a) and porcine circovirus type 2b (PCV-2b). Each target produced specific amplicon with a size of 229 bp (PCV-2a) and 785 bp (PCV-2b). The assay was sensitive and specific in detecting the target agent in clinical specimens. In conclusion, the PCR has the potential to be useful for routine molecular diagnosis and epidemiology.
ER  -