TY  - JOUR
T1  - <I>In vitro</I> Pharmacodynamics of Enrofloxacin Against an <I>Escherichia coli gyrA</I> Mutant
AU - Sorucu, Ali AU - Arslan, Erdem AU - Cengiz, Murat 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 18
SP  - 2384
EP  - 2388
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2384.2388
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2384.2388
KW  - Turkey
KW  -Pharmacodynamic of enrofloxacin
KW  -Escherichia coli
KW  -C-T mutation
KW  -microdilution
KW  -significant
AB  - The aim of this study was to investigate <I>in vitro</I> the pharmacodynamics of Enrofloxacin (ENR) against an <I>Escherichia coli gyrA</I> mutant (<I>E. coli </I>MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) amd multi-step resistance selection was performed until reaching 1 &#956;g mL&#8226;<SUP>1</SUP> MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for <I>E. coli </I>MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations &#8226; 8 times the MIC with no significant regrowth by 24 h.
ER  -