TY  - JOUR
T1  - Rescue of Duck Hepatitis Virus-1 from Cloned cDNA by Using T7 RNA Polymerase
AU - Yu, M. AU - Ning, Z.Y. AU - Cao, Z.X. AU - Li, R.S. AU - Huang, C.L. AU - Zhang, G.H. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 18
SP  - 2372
EP  - 2375
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2372.2375
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2372.2375
KW  - Duck hepatitis virus type 1
KW  -infectious cDNA
KW  -rescue system
KW  -competent
KW  -rescaed viras
KW  -China
AB  - In the present study, researchers describe a rescue system for duck hepatitis virus-1 using T7 RNA polymerase. This strain can not induce typical cytopathic effect in BHK-21 cells. First, there is used the vitro transcripts to infect BHK-21 cells. Then the infected cell supernatant were inoculated into chicken embryos to rescue the virus and the cDNA clone was engineered to contain one silent nucleotide change to create a BssHII site to distinguish from the parental virus. The rescued virus was able to result in embryonic death and have similar infection with parental virus in chicken embryos indicating that the cDNA clones were replication competent. It is proposed that this rescue system may benefit the investigation on viral virulence determinants and molecular virology of DHV-1 virus.
ER  -