TY  - JOUR
T1  - Development and Application of the Novel Visual Loop-Mediated Isothermal Amplification of Omp25 Sequence for Rapid Detection of <I>Brucella</I> sp.
AU - Zhao, Ming-Qiu AU - Chen, Jin-Ding AU - Dong, Xiao-Ying AU - Yi, Lin AU - Ju, Chun-Mei AU - Shen, Hai-Yan AU - Wang, Jia-Ying AU - Pan, Wen 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 16
SP  - 2120
EP  - 2126
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2120.2126
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2120.2126
KW  - food
KW  -detection
KW  -omp25 gene
KW  -visual LAMP
KW  -Brucella sp
KW  -China
AB  - A novel visual Loop-mediated isothermal Amplification (LAMP) method for detecting <I>Brucella</I> sp. was established. LAMP primers were designed by targeting the species-specific <I>omp25</I> gene. In this LAMP reaction, manganous ion and calcein were added which allows the results to be simply judged by naked eye under daylight by color change from orange to green in a closed-tube system without expensive equipment and it took &lt;60 min at 63&deg;C. The assay correctly identified 3 <I>Brucella</I> sp. strains but did not detect 8 other bacterial species. Detection limit of genomic DNA of<I> Brucella</I> sp. was 10 pg. The assay was also successfully applied for the detection of <I>Brucella</I> sp. from contaminated milk (as low as 1.3x10<SUP>3 </SUP>CFU mL<SUP>-1</SUP>) and infected mice tissues. The assay shows enormous potential for rapidly, expediently and accurately detecting <I>Brucella</I> sp. from food and clinical samples.
ER  -