TY  - JOUR
T1  - A Simpler Method for the Efficient and Precise Deletion of Genes in<I> Salmonella </I>sp.
AU - Wen, Zhi-Fa AU - Chen, Xiang AU - Geng, Shi-Zhong AU - Fang, Qiang AU - Pan, Zhi-Ming AU - Jiao, Xin-An 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 16
SP  - 2090
EP  - 2094
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2090.2094
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2090.2094
KW  - temperature
KW  -Salmonella
KW  -deletion of gene
KW  -prgH genehomologous recombination
KW  -China
AB  - A simpler method for the efficient and precise deletion of genes in <I>Salmonella </I>sp. was developed. To demonstrate this approach, the <I>prgH </I>gene of <I>Salmonella typhimurium </I>SL7207 was deleted by homologous recombination with a temperature-sensitive plasmid containing a cassette that two DNA fragments as homologous arms flanking chloramphenicol resistance gene (<I>Cm<SUP>R</SUP></I>) which replaced the <I>prgH </I>gene. During screening mutant at 44&deg;C, the temperature-sensitive plasmid was lost easily only the mutant which<I> prgH</I> gene was replaced by <I>Cm<SUP>R</SUP></I> gene could grow in the LB media with chloramphenicol. The results showed that the method was simpler, more effective to delete target gene in genomic DNA of<I> Salmonell </I>sp. than those conventional methods.
ER  -