TY  - JOUR
T1  - Polymorphism Analysis of the Horse Dopamine Receptor D4 Gene <I>(DRD4) </I>Sequence
AU - Manglai, Dugarjaviin AU - Wang, Qinghua AU - Li, Jinlian AU - Cheng, Jianbo AU - Fan, Caiyun 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 14
SP  - 1855
EP  - 1858
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1855.1858
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1855.1858
KW  - PCR-RFLP
KW  -polymorphism
KW  -DRD4 gene
KW  -China
KW  -local bread
KW  -silver staining
KW  -Horses
AB  - In this study, partial sequence of horse <I>DRD4 </I>gene was cloned including intron 1, partial exon 1 and exon 2. And restriction endonuclease Stu I was used to analyze the polymorphism of the <I>DRD4 </I>gene sequences of 270 horses from six types including importing breed, cultivating breed and local breed. The products of endonuclease cutting were detected by 8% non-denatured polyacrylamide gel electrophoresis and showing in silver staining protocol.The result indicated restriction endonuclease Stu I showed polymorphism. Six kinds of genotypes were found in six populations which were controlled by three alleles. The results of Chi-square (&#967;<SUP>2</SUP>) test showed that genotypes of horse <I>DRD4 </I>gene in TB, SH, XN did not fit with Hardy-Weinberg equilibrium (p&lt;0.05) but in WS, BH and WZ fit with Hardy-Weinberg equilibrium (p&gt;0.05).
ER  -