TY  - JOUR
T1  - Effects of Interferon-Tau (IFN-τ) and Progesterone on Transcription Level of Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) in Cultured Bovine Endometrial Cells
AU - Lai, Song-Jia AU - Wang, Jie AU - Wang, Wei AU - Chen, Si-Yi AU - Yao, Jia AU - Zhang, Ming AU - Feng, Yu-Mei 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 14
SP  - 1833
EP  - 1840
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1833.1840
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1833.1840
KW  - bovine endometrium
KW  -TIMP-2
KW  -progesterone
KW  -IFN-Ä
KW  -MMP-2
KW  -China
AB  - Interferon-tau (IFN-&#964;) secreted by blastular trophectoderm was thought to be the primary signal for pregnancy recognition during bovine early pregnancy period after pregnancy recognition, progesterone dominated pregnancy maintaining. Moreover, Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) was important for embryo implantation. The objectives of studies were evaluated whether IFN-&#964; and progesterone regulated MMP-2 and TIMP-2 expression in cultured bovine Endometrial Cells (bECs). In the studies, bECs were cultured in DMEM-Ham&#146;s F12 medium with free-serum. These cultured cells were divided into 4 groups and cultured in four types culture medium: culture medium without IFN-&#964; and progesterone (Co group); culture medium including 100 ng mL<SUP>-1</SUP> IFN-&#964; (I<SUB>N</SUB> group); culture medium including 200 nmol L<SUP>-1</SUP> progesterone (Pr group); culture medium including 100 ng mL<SUP>-1</SUP> IFN-&#964; and 200 nmol L<SUP>-1</SUP> progesterone (I+P group). Cultured bECs were harvested and extracted total mRNA after being cultured for 1, 3, 6 and 12 h and then expression level of MMP-2 and TIMP-2 were assessed with fluorescence quantitative PCR. The results showed that MMP-2 expression level in Pr, I<SUB>N</SUB> and I+P group were extremely significantly higher than in Co group (p&lt;0.01). MMP-2 expression level in I<SUB>N</SUB> and I+P group was significantly higher than in Pr group (p&lt;0.05). MMP-2 expression level was no statistical difference between I<SUB>N</SUB> group and I+P group (p&gt;0.05); TIMP-2 expression level in Pr, I<SUB>N</SUB> and I+P group was extremely significantly lower than in Co group (p&lt;0.01) and TIMP-2 expression level was no statistical difference among Pr, I<SUB>N</SUB> and I+P group (p&gt;0.05). The conclusion indicated progesterone and IFN-&#964; induced MMP-2 expression <I>in vitro</I> cultured bECs whereas, progesterone and IFN-&#964; suppressed TIMP-2 expression <I>in vitro</I> cultured bECs.
ER  -