TY  - JOUR
T1  - Plasmid Analysis of Fluoroquinolone Resistant Commensal <I>E. coli </I>from Faecal Samples of Apparently Healthy Cattle in Ado-Ekiti, Ekiti-State
AU - Famurewa, O. AU - Olowe, O.A. AU - Ajayi, A.O. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 2
SP  - 180
EP  - 184
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.180.184
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.180.184
KW  - plasmid
KW  -fluoroquinolone resistance
KW  -Prevalence
KW  -eosine
KW  -phenotype
KW  -Nigeria
AB  - This study was carried out to investigate the prevalence of fluoroquinolone resistance and plasmid carriage among isolates of commensal <I>E. coli </I>isolated from faeces of cattle. Fresh faecal samples were collected from apparently healthy cattle and were cultured on eosine methylene blue agar plates from which 500 commensal <I>E. coli </I>isolates were recovered and characterised using standard biochemical tests. Using protocol recommended by the Clinical Laboratory Science Institute, all isolates were examined for their susceptibility to five fluoroquinolones: norfloxacine (5 &#956;g), levofloxacine (5 &#956;g), pefloxacine (5 &#956;g), ofloxacine (5 &#956;g) and ciprofloxacine (5 &#956;g). The resistance among isolates against the fluoroquinolones are as follows: pefloxacine, 99 (19.8%); ciprofloxacine, 55 (11.0%); norfloxacine, 39 (7.5%); ofloxacine 26 (5.2%) while the isolates showed least resistance against levofloxacine 23 (4.6%). The organisms also showed considerable multiple fluoroquinolone-resistance and sixteen different fluoroquinolone-resistance phenotypes were observed with the most prominent phenotype observed to be Cip-Nor-Ofx-Pef-Lev. Thirteen representative isolates were selected and examined for the presence of plasmids. Twelve of the representative isolates carried multiple plasmids while one isolate carried a single plasmid. After mating experiments, plasmids were transferred to recipient strains at high frequencies of conjugation. These findings have serious public health implications as fluoroquinolone-resistant bacteria could be shed into the immediate environments, food and drinking water sources.
ER  -