TY  - JOUR
T1  - Serotyping <I>Dichelobacter nodosus</I> with PCR-SSCP
AU - Hickford, Jon AU - Cagatay, Tulay Ifakat 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 13
SP  - 1678
EP  - 1682
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1678.1682
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1678.1682
KW  - Single Strand Conformational Polymorphism (SSCP)
KW  -PCR
KW  -fimA gene
KW  -Dichelobacter nodosus
KW  -sequencing
KW  -sample
AB  - In this study, we reported rapid approach for identification of <I>D. nodosus</I> serotypes that causes footrot, higly contagious diseases of the ruminants in worldwide with using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) analysis. A total of 500 swab samples from infected hoof lesion were investigated for presence of <I>D. nodosus</I> by PCR amplification of <I>fimA</I> gene. PCR-SSCP patterns revealed that each sample contained two or three different <I>D. nodosus</I> serotypes. Additionally on the of basis SSCP patterns, 8 different serogroups (A- G and I) were identified from New Zealand samples. These findings were confirmed by cloning and sequences of <I>fimA</I> gene.
ER  -