TY  - JOUR
T1  - Polymorphisms and Sequencing Analysis in 5'Upstream and Intron 2 Region of <I>H-FABP</I> Gene in Anhui Wannan Black Pig Population
AU - Yin, Zongjun AU - Zhang, Chenghua AU - Ding, Xiaoling AU - He, Xiaolei AU - Huang, Long AU - Ding, Yueyun 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 12
SP  - 1568
EP  - 1572
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1568.1572
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1568.1572
KW  - sequencing
KW  -China
KW  -PCR-RFLP
KW  -IMF content
KW  -H-FABP
KW  -Wannan black pig
AB  - In order to give a basis for molecular breeding of meet quality traits in Wannan black pig, we applied PCR-RFLP method to analyze the polymorphism of 5'upstream region and intron 2 of <I>H-FABP</I> gene in 184 Wannan black pigs population. The results show that there exist polymorphic loci of restriction enzyme Hinf in 5'upstream region and Hae III as well as Hinf I in intron 2. Except for locus of restriction enzyme HinfI, the other two polymorphism sites achieved in Hardy-Weinberg equilibrium based on &#967;<SUP>2</SUP>-test (p&gt;0.05). In addition, a T&rarr;C mutation in 1324 bp, a C&rarr;G mutation in 1811 bp and a C&rarr;T mutation in 1970 bp were found through the direct sequencing the polymorphic RFLP fragments of <I>H-FABP</I> gene, then genotype frequencies, gene frequencies, H<SUB>o</SUB>, H<SUB>e</SUB>, N<SUB>e</SUB> and PIC were investigated in Wannan black pig population. The results identified 3 variant restriction sites and provided a basic data for further study of the relationship between IMF and H-FABP with different genotypes and also provide basic for make sure whether <I>H-FABP</I> gene is the key gene for effect on the deposition of IMF.
ER  -