TY  - JOUR
T1  - The Association of ANKRD2 with Loin Depth and Muscle Firmness in Pigs
AU - Liu, Bang AU - Sun, Ling AU - Dong, Xinjie AU - Fan, Bin 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 11
SP  - 1462
EP  - 1468
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1462.1468
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1462.1468
KW  - ANKRD2
KW  -meat quality trait
KW  -expression profile
KW  -association analysis
KW  -Sequence analysis
KW  -carcass trait
AB  - The Muscle Ankyrin Repeat Proteins (MARPs) family is essential for cardiogenesis and Cardiac hypertrophy (CARP), skeletal muscular stretch and development (ANKRD2/ARPP) and during recovery following starvation (DARP). Here we describe the isolation and molecular characterization of the full-length cDNA of porcine gene <I>ANKRD2 </I>(ankyrin repeated domain 2). The cDNA sequence and predicted protein sequence shared high sequence identity with other mammalian counterparts. The Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) revealed that ANKRD2 has a high level of expression in skeletal muscle and a low expression in heart while the other tissues being tested had no expression. The expression of <I>ANKRD2</I> gene was up-regulated accompanying with the different embryo developmental stages (embryo 33, 65, 90 days; postnatal 2 days, adult) and reached the peak at the adult time. A T/C single nucleotide polymorphism in ANKRD2 exon5 was detected and a HaeIII PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) was then developed. Association of the genotypes with growth and carcass traits revealed significant associations of the porcine ANKRD2 loin depth (p&lt;0.05) and firmness (p&lt;0.05).
ER  -