TY  - JOUR
T1  - Some Properties of a New Thermostable Xylanase from Alkaliphilic and Thermophilic <I>Bacillus</I> sp. Isolate DM-15
AU - Ozcan, Bahri Devrim AU - Coskun, Ali AU - Ozcan, Numan AU - Baylan, Makbule 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 2
SP  - 138
EP  - 143
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.138.143
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.138.143
KW  - molecular weight
KW  -Bacillus sp
KW  -characterization
KW  -Thermostable xylanase
KW  -optimum
KW  -thermal spring
AB  - Alkaliphilic and thermophilic <I>Bacillus</I> sp. DM-15 was isolated from ciftehan thermal spring, Turkey, produced thermostable xylanase at 55&deg;C at pH 9. The molecular weight of the enzyme was estimated to be 95.6 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The enzyme showed broad working pH range of 4.5-10.0 with an optimum pH of 6.5. The temperature optimum of the enzyme was found to be 60-70&deg;C. Xylanase production by thermophilic <I>Bacillus</I> sp. strain cultivated in liquid media reached a maximum at 36 h, with levels of 192.8 &#956;mol mg<SUP>-1</SUP> protein/min. The enzyme was stable for 15 min at 60&deg;C while 13, 86, 95 and 96% of the original activities were lost at 70, 80, 90 and 100&deg;C, respectively. Enzyme activity was increased in the presence of 5 mM CaCl<SUB>2</SUB> (137%), CoCl<SUB>2</SUB> (133%), CuSO<SUB>4</SUB> (112%), MgCl<SUB>2</SUB> (106%) and KCl (105%) and inhibited in the presence of 5 mM EDTA, HgCl<SUB>2</SUB>, FeSO<SUB>4</SUB>, SDS (1%), MnCl<SUB>2</SUB>, Triton X-100 (1%) up to 93, 89, 73, 41, 29 and 8%, respectively. The DM-15 thermostable xylanase may be suitable for several industrial applications, such as food, agricultural and biofuel.
ER  -