TY  - JOUR
T1  - Genetic Characterization of Porcine Circovirus Type 2 (PCV2) from Pigs in Auhui Province
AU - Jian-Zhong, Wei AU - Qian, Xie AU - Pei, Sun AU - Da-Wen, Xu AU - Rong, Chen AU - Yu, Li AU - Zong-Jun, Yin 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 8
SP  - 1024
EP  - 1031
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1024.1031
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1024.1031
KW  - variation
KW  -sequence analysis
KW  -ORF2
KW  -whole genomic sequence
KW  -PCV2
KW  -regions
AB  - To understand the molecular characteristics in PCV2 strains from Anhui province in China, the whole genomic sequences of the 6 PCV2 Anhui isolates which were associated with PMWS were cloning, sequencing and analyzing. The results show that full genomic sequences of all of 6 PCV2 Anhui isolates were 1767 nt in length and belong to the different clusters in the PCV2b, PCV2-1A, PCV2-1B, PCV2-1C. The genome of 6 PCV2 Anhui isolates have 99.5-99.7% homology in whole nucleotide sequence have 97.2-99.8% homology in ORF1 nucleotide sequence and 93.6-99.9% homology in ORF2 nucleotide sequence and have 97.0-100% homology in ORF2 amino acid sequence. About 6 PCV2, Anhui isolates have the 93-99.5% homology in the whole nucleotide sequence, 96.2-100% in ORF1, 89.8-100% in ORF2 with 42 strains of domestic and foreign. About 30 PCV2b isolates having the identity of 93.2-100% in ORF2 nucleotide sequence and 91.0-100% in ORF2 amino acid sequence. Researchers conclude that PCV2 genome nucleotide sequences were more stable and do not have stronger variations of different characteristics with the change of time and regions. PCV2 strains in Anhui region are close relatives but showed some of the regional characteristics; the variation was mainly concentrated in PCV2 ORF2. PCV2-1C gene cluster have changed in the ORF2 of the two main strains of the immune response zone.
ER  -