TY  - JOUR
T1  - Introduction of Exogenous Gene into Chicken PGCs via Blastoderm
AU - Yoshida, T. AU - Takayanagi, R. AU - Sanada, T. AU - Furuta, H. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 4
SP  - 748
EP  - 750
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.748.750
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.748.750
KW  - blastoderm
KW  - PGCs
KW  -gene transfer
KW  -Chicken embryo
KW  -exogenous gene
KW  -Lac Z
AB  - It was proved that an exogenous gene was successfully transferred to chicken embryo and Primordial Germ Cells (PGCs) by the injection of the blastoderm. In this experiment, an exogenous gene, Lac Z constructs encoding <I>Escherichia coli</I> beta-galactosidase was introduced into chicken blastoderm (stage X) just ovoposition using lipofection reagent. After injection of gene the eggs were incubated in routine manner until developmental stage 12-15. Survival rate of the treated embryo was 35.6%. Lac Z expression was mosaic manner and low efficiency. However, it was obtained Lac Z specific band from chicken PGCs by PCR method.
ER  -