TY  - JOUR
T1  - Field Evaluation of PCR Assays for the Diagnosis of Tropical Theileriosis in Cattle and Water Buffaloes in Egypt
AU - Zhu, X.Q. AU - Monazie, A.M. AU - El-Mekkawy, M.F. AU - Mahmmod, Y.S. AU - El-Balkemy, F.A. AU - Yuan, Z.G. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 4
SP  - 696
EP  - 699
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.696.699
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.696.699
KW  - Theileria annulata
KW  -tropical theileriosis
KW  -PCR
KW  -microscopic examination
KW  -diagnosis
KW  -Cattle
KW  -water buffaloes
KW  -Egypt
AB  - The study evaluated the usefulness of specific PCR assays for the diagnosis of tropicaltheileriosis caused by <I>Theileria annulata</I> in Egypt using Microscopic Examination (ME) for comparison. Blood samples from 258 animals comprising both sick and apparently healthy cattle and water buffaloes were examined for the presence of <I>Theileria</I> infection by ME using Giemsa-stained blood smears and the prevalence was 18.6% (48/258). To evaluate the usefulness of PCR assays for the identification <I> Theileria</I> sp. and <I>T. annulata</I>, 30 bovine blood samples selected from the 258 animals were tested by both PCR assays and ME and ME identified 9/30 (30%), whereas the PCR assay for <I>Theileria</I> sp. was more sensitive which identified 21/30 (70%). Of these 21 <I>Theileria</I> positive samples, 15 were identified as infected with <I>T. annulata</I> by specific PCR assay for <I>T. annulata</I>. This study demonstrated that specific PCR assays are more sensitive and accurate for the clinical diagnosis of tropical theileriosis.
ER  -