TY  - JOUR
T1  - Identification and Sequencing of Two Expression Genes of Porcine <I>Pasteurella multocida in vivo</I>
AU - Wen, Xintian AU - Zou, Nianli AU - Cao, Sanjie AU - Huang, Yong AU - Tian, Mingxing AU - Huang, Xianqi 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 3
SP  - 441
EP  - 445
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.441.445
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.441.445
KW  - identification
KW  -in vivo-expressed gene
KW  -Pasteurella multocida
KW  -cloning
KW  -sequencing
KW  -RT-PCR
AB  - </B>According to the published sequence of 14 avian <I>Pasteurella multocida</I> genes required for survival and expression <I>in vivo</I>, the swine<I> P. multocida</I> infected models in septicemic mice were established and 14 pairs of primers were designed and synthesized to amplify the corresponding gene of swine <I>P. multocida </I>CVCC 432 strain. Successfully, two genes, pm0221 and pm1069 were identified as <I>in vivo</I>-expression gene using RT-PCR. Their nucleotide sequences were compared with those of avian <I>P. multocida </I>pm70 strain and the homology were 98.8 and 94.8%, respectively.
ER  -