TY  - JOUR
T1  - Detection of <I>Mycobacterium avium</I> Subspecies <I>paratuberculosis</I> in Milk and fecal Samples in Dairy Cattle by PCR and Nested-PCR
AU - Badiie, A. AU - Moghadam, M. Tohidi AU - Sarv, S. AU - Moosakhani, F. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 24
SP  - 3055
EP  - 3061
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.3055.3061
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.3055.3061
KW  - feces
KW  -single PCR
KW  -paratuberculosis
KW  -milk
KW  -Mycobacterium avium
KW  -subsp
KW  -nested-PCR
KW  -Cattle
AB  - <I>Mycobacterium avium</I> ssp. <I>paratuberculosis</I> (MAP) is the etiologic agent of Johne&#146;s disease in cattle. The disease causes diarrhea, reduced milk production, poor reproductively, infertility and emaciation. MAP was isolated from intestinal tissue of a human patient suffering from Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Milk may be a potential vehicle for the transmission of MAP to human population. In this study, milk and fecal samples were collected from cows (n = 150) with suspected of Johne&#146;s status. Milk and fecal samples were processed for Nested PCR. Nested PCR detected positive samples in milk and feces 68.150 (45.3%) and 74.150 (49.3%), respectively and PCR detected positive samples in milk and feces 41.150 (27.3%) and 48.150 (32%), respectively.
ER  -