TY  - JOUR
T1  - Expression of Interferon-Stimulated Gene 15 in Endometrial Explants from Early Pregnant Cows <I>in vitro</I> for 60 Days
AU - Zhang, Le-Ying AU - Li, Jian-Guo AU - Yang, Ling AU - Wang, Bin 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 24
SP  - 3016
EP  - 3020
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.3016.3020
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.3016.3020
KW  - ISG1 5
KW  -primary experiment
KW  -in vitro
KW  -bovine
KW  -Endometrium
KW  -pregnant cow
AB  - Interferon-tau is generally regarded as the primary signal for maternal recognition in ruminant animals. Expression of interferon-stimulated gene 15 (ISG15) is up-regulated in bovine endometrium during early pregnancy. The experimental materials were from our primary experiment. Tissue explants of endometria from day 18 of pregnant and non-pregnant cows were cultured <I>in vitro</I> for 60 days and then the expression of ISG15 in these explants was detected by western blot. The results indicated that ISG15 and conjugated proteins were still expressed at a high level in endometrial explants from pregnant cows after cultured <I>in vitro</I> for 60 days and there was no expression in endometrial explants from non-pregnant cows after cultured <I>in vitro</I> for 60 days. In conclusion, expression of ISG15 in endometrial explants from early pregnant cows maintained <I>in vitro</I> for a long time.
ER  -