TY  - JOUR
T1  - Effect of Roscovitine Pretreatment on the Meiotic Maturation of Bovine Oocytes and their Subsequent Development after Somatic Cell Nuclear Transfer
AU - Kaedei, Yukine AU - Fujiwara, Akira AU - Ito, Aya AU - Tanihara, Fuminori AU - Morita, Yasuhiro AU - Hanatate, Keisuke AU - Viet, Vien Luu AU - Namula, Zhao AU - Otoi, Takeshige 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 22
SP  - 2848
EP  - 2853
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.2848.2853
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.2848.2853
KW  - Meiotic inhibition
KW  -prematuration
KW  -short storage
KW  -nuclear transfer
KW  -polar body
KW  -SCNT
AB  - Roscovitine, a specific inhibitor of M-phase promoting factor kinase activity was used to inhibit the completion of meiotic maturation of bovine oocytes. The objectives of this study were to evaluate the nuclear maturation of bovine oocytes pre-cultured with various concentrations (0, 50, 100 and 200 &#956;M) of roscovitine before <I>in vitro</I> Maturation (IVM) and to examine the development of Somatic Cell Nuclear Transfer (SCNT) embryos derived from the oocytes pre-cultured with roscovitine. Before IVM, 72% of oocytes that were cultured without roscovitine (control) had reached the Metaphase II (MII) stage whereas culture with roscovitine decreased the rates of oocytes reaching MII (11-27%). After IVM, the maturation rate of oocytes pre-cultured with 200 &#956;M roscovitine was significantly higher than that of control oocytes (79 vs. 58%). Moreover, significantly more oocytes extruded the first polar body in the 50 &#956;M roscovitine group than in the control group (64 vs. 51%). The rate of blastocyst formation of reconstructed embryos derived from oocytes pre-cultured with 50 &#956;M roscovitine was significantly higher than that from the control oocytes (14 vs. 6%). In this study, the addition of roscovitine to culture medium delays the completion of meiotic maturation of bovine oocytes and the cytoplasm derived from oocytes pre-cultured under meiotic inhibition can support the development of SCNT embryos.
ER  -