TY  - JOUR
T1  - Prolactin Genotyping of Najdi Cattle Breed Using PCR-RFLP
AU - Sharifi, S. AU - Roshanfekr, H. AU - Khatami, S.R. AU - Mirzadeh, K.H. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 2
SP  - 281
EP  - 283
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.281.283
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.281.283
KW  - Prolactin gene
KW  -exon III
KW  -Najdi cows
KW  -PCR-RFLP
KW  -RsaI
KW  -genotype
AB  - Prolactin is a peptide hormone synthesized by lactotropes of the anterior pituitary. prolactin plays an important regulatory function in milk production and reproduction. Genomic DNA was isolated from blood samples of 84 Najdi cattle. A 156 bp PRL gene exon III segment was amplified by PCR using bovine specific primers. RFLPs in this segment was studied using RsaI restriction enzyme. The frequencies of genotypes were as follows: 0.2857-AA, 0.5714-AB, 0.1429-BB; Frequencies of allele A and B were 0.571 and 0.429 in Najdi Cows.
ER  -