TY  - JOUR
T1  - Using Bioinpormcotics Methods to Develop EST-SSR Makers from Sheep’s ESTs
AU - Zhang, Wenxiang AU - Wang, Zunbao AU - Zhao, Zongsheng AU - Zeng, Xiancun AU - Wu, Hongbin AU - , Peng Yu 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 21
SP  - 2759
EP  - 2762
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.2759.2762
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.2759.2762
KW  - Sheep
KW  -EST
KW  -SSR
KW  -NCBI
KW  -genetic mapping
KW  -China
AB  - About 29685 ESTs of sheep’s skin from NCBI were mined to develop EST-SSR markers. Totally, 1456 SSRs were identified from 1364 ESTs using SSRHunter soft tools. The frequency of EST-SSRs was 4.9%. The dinucleotide repeat motif was the most abundant SSR accounting for 32.14% followed by 28.23, 7.14, 25.21 and 7.28%, respectively for tri, hexa, penta and tetra-nucleotide repeats. Among the di-nucleotide repeats, AC/TG (58.33%) were the most abundant type. About 261 primer pairs were designed from microsatellite among which 62 pairs could show clear PCR products by electrophoresisand 5 of these primers revealed moderate to high Polymorphism Information Content (PIC) in Chinese Merino sheep. The mean PIC value was 0.6971, ranging from 0.5499-0.7440.
ER  -