TY  - JOUR
T1  - The Polyclonal Antibody Against Chicken Interleukin-6 Prepared by Immunization of Recombinant Eukaryotic Plasmid Can React Efficiently with its Prokaryotic Expression Protein
AU - Li, Min AU - Wang, Yuanping AU - Zou, Nianli AU - Cao, Sanjie AU - Wen, Xintian AU - Huang, Yong 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 21
SP  - 2679
EP  - 2684
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.2679.2684
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.2679.2684
KW  - Chicken IL-6
KW  -eukaryotic expression
KW  -prokaryotic expression
KW  -polyclonal antibody
KW  -Western-blot
KW  -indirect ELISA
AB  - Interleukin-6 (IL-6) is a multifunctional cytokine which has a wide range of activity in animals and has an impact on almost all immune system cells. To produce specific antibody against chicken IL-6 and detect its activity, its complete Open Reading Frame (ORF) sequence was amplified and inserted into the eukaryotic expression vector pcDNA3.1 (+) to get recombinant eukaryotic plasmid pcDNA-ChIL-6 and BALB/c mouse were immunized with this plasmid to produce polyclonal antibody against Chicken IL-6. Then the mature protein coding genes of chicken IL-6 was cloned and inserted into the prokaryotic expression vector pET-32a (+) to get recombinant prokaryotic plasmid pET-ChIL-6, the expression of this fusion protein was successfully induced by Isopropy 1-β-D-thiogalactoside (IPTG) and the recombinant protein was further purified by NTA His•Bind column. The purified recombinant protein could react positively with polyclonal antibody against pcDNA-ChIL-6 in Western-blot and indirect ELISA.
ER  -