TY  - JOUR
T1  - Investigation of Growth Hormone Gene Polymorphism Using PCR-RFLP Technique in Native Poultry in Khouzestan Province
AU - Alireza, Darabi AU - Jamal, Fayazi AU - Hedayatolla, Roshanfekr AU - Taghi, Nasiri Mohammad 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 2
SP  - 255
EP  - 257
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.255.257
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.255.257
KW  - Growth hormone
KW  -heterozygosity
KW  -native chicken
KW  -polymorphism
KW  -gene
KW  -genotype
AB  - Native animal and poultry in every province are valuable genetic resourses. Khouzestan province native poultry is one of the resourses that bacuase of adaptating to environment. The growth hormone gene is a candidate genes controling the metabolic and physiologic actions. Polymorphism within this gene is a detectable parameter. In order to study and investigate growth hormone gene polymorphism, blood samples were collected from 100 individuals of native poultry and genomic DNA was extracted using salting out technique. DNA fragment with 776 bp at growth hormone gene was amplified using Polymerase Chain Reaction (PCR). The PCR product was digested with restriction enzymes MspI and subsequently electroforesed on 2.5% agarose gel with etidium bromide. The studied intron in this research was at Hardy-Weinberg disequilibrium (p<0.005). In this population, heterozygosity and effective allele were estimated 0.59 and 2.13, respectively. Obtained genotypes frequency of MspI enzyme on 776 bp fragment for AA, AB and BB genotypes were 0.39, 0.59 and 0.02, respectively. The frequency of A and B alleles were 0.685 and 0.315, respectively.
ER  -