TY  - JOUR
T1  - Sequences of Internal Transcribed Spacers and Two Mitochondrial Genes: Effective Genetic Markers for <I>Metorchis orientalis</I>
AU - Zhu, X.Q. AU - Cai, Y.C. AU - Guo, J. AU - Chen, M.X. AU - Li, H. AU - Zhou, X.N. AU - Chen, S.H. AU - Zhang, Y.N. AU - Ai, L. AU - Chen, J.X. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 18
SP  - 2371
EP  - 2376
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.2371.2376
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.2371.2376
KW  - cytochromec oxidase subunit 1 (cox1)
KW  -mitochondrial DNA (mtDNA)
KW  -Internal Transcribed Spacers (ITS)
KW  -trematodes
KW  -Opisthorchiidae
KW  -metacercaria
KW  -Metorchis orientalis
KW  -NADH dehydrogenase subunits 1 (nad1)
KW  -phylogenetic analysis
KW  -genetic marker
AB  - The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) and two mitochondrial DNA (mtDNA) regions, namely cytochromec oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 (nad1), among <I>Metorchis orientalis</I> metacercaria isolates from Guangxi in China. The sequences of ITS, pcox1 and pnad1 were amplified from 6 individual <I>M. orientalis</I> metacercariae and sequenced. The relevant sequences of other 7 trematode species belonging to 6 genera in 4 families were downloaded from GenBank and their phylogenetic relationships were re-constructed using the combined pcox1 and pnad1 mt DNA sequences with <I>Trichinella spiralis</I> as outgroup. The results showed that sequences of ITS rDNA, pcox1 and pnad1 of <I>M. orientalis</I> were 1131, 654 and 650 bp, respectively and they were quite conserved among the <I>M. orientalis</I> isolates. However, they were quite different from that of other species, phylogenetic analysis of the combined pcox1 and pnad1 mt DNA sequences were able to distinguish <I>M. orientalis</I> from different species of the <I>Opisthorchiidae</I> and trematodes in other families. Therefore, the ITS, cox1 and nad1 mt DNA sequences provide effective genetic markers for the specific identification of trematodes of the <I>Opisthorchiidae</I> family and have implications for studying their population biology, genetic structure, as well as molecular epidemiology.
ER  -