TY  - JOUR
T1  - Molecular Characteristics of the Porcine <I>MUSTN1</I> Gene and its Significant Association with Economic Traits
AU - Liu, B. AU - Xu, X.W. AU - Han, X.L. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 18
SP  - 2351
EP  - 2356
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.2351.2356
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.2351.2356
KW  - association analysis
KW  -SNP
KW  -MUSTN1
KW  -Pig
KW  -meat quality traits
KW  -expression
AB  - The <I>MUSTN1</I> (musculoskeletal, embryonic nuclear protein 1) gene plays an important role in the development and regeneration of the mammalian musculoskeletal system and has a high expression in the muscle. In the present study, we cloned the porcine <I>MUSTN1</I> genome DNA sequence including the full-length cDNA. Analysis of the genomic sequence revealed that porcine <I>MUSTN1</I> consists of 3 exons separated by 2 introns. It contains a 237 bp open reading frame flanked by 251 bp 5&#146;UTR and 216 bp 3&#146;UTR. Radiation hybrid mapping data indicated that the porcine <I>MUSTN1</I> gene was located to chromosome 13q12-q22. The expression data revealed that the porcine <I>MUSTN1</I> gene is widely expressed in the examined tissues and has an especially high expression level in muscle tissues. One single nucleotide polymorphism c. 265C &gt;T was identified in the 3&#146;UTR of the porcine <I>MUSTN1</I> gene and genotyped by the NruI PCR-RFLP. Association analysis showed that different genotypes of the <I>MUSTN1</I> gene were significantly associated with ADG (average daily gain from birth to 100 kg), percentage of ham, proportion of lean and bone of the ham and meat color (p&lt;0.05). Thus, the mutation can be applied to pig breeding by molecular Marker-Assisted Selection (MAS).
ER  -