TY  - JOUR
T1  - The Effects of IFN-γ on Expression of IL-18 in Uterus and Ovaries and IL-18 Levels in Peripheral Blood of Abortion Rats
AU - Li-Fang, Si AU - Shu-Lin, Chen AU - Xiang-Chao, Cheng AU - Su-Zhu, Qing AU - Xian-Li, Si AU - Xiao-Ying, Zhang AU - Jin-Yi, Wang AU - Yong-Ping, Xu AU - Guang-Li, Fan 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 16
SP  - 2194
EP  - 2199
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.2194.2199
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.2194.2199
KW  - IL-18
KW  - IFN-y
KW  -uterus
KW  -ovary
KW  -abortion
KW  -rat
AB  - To explore the immunological regulatory mechanisms of IFN-γ on IL-18 and its effect on pregnancy, the dynamic expression of mRNA and cellular localization of protein for IL-18 in the early pregnant rats after the injection of different doses of IFN-γ were observed by RT-PCR and immunohistochemistry. ELISA assay was also applied to investigate the effect of exogenous IFN-γ on the level of IL-18 in the peripheral blood. The results indicated that IL-18 mRNA expression level was the highest in uterus and ovaries of normal pregnancy group and the lowest in abortion model group; compared with the abortion model group, the IL-18 mRNA expression level was up-regulated both in 2.4 and 2.8 IU IFN-γ dose group. Howere, no significant difference can be seen between 2 IU IFN-γ dose group and the normal pregnancy group (p>0.05). Furthermore, IL-18 protein was strongly expressed in the normal pregnant group and 2IU IFN-γ group. ELISA results showed that the level of IL-18 in peripheral blood of abortion model was the lowest and it was extremely significantly different from normal pregnancy group and 2 IU IFN-γ group (p<0.01).
ER  -