TY  - JOUR
T1  - The Study of <I>PIT1</I> Gene Polymorphism in the Najdi Cattle Using PCR-RFLP Method
AU - Tavakoli, S. AU - Fayazil, J. AU - Hartatik, T. AU - Biranvand, Z. AU - Beigi Nassiri, M.T. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 15
SP  - 2001
EP  - 2003
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.2001.2003
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.2001.2003
KW  - growth harmone
KW  -allele
KW  -najdi cattle
KW  -polymorphism
KW  -PIT1 gene
KW  -prolactin
AB  - The part of the bovine genome which shows a superior action and explains the major part of variation of the economical production traits were known as QTL. <I>PIT1</I> gene which is also termed hormone factor-1 is a pituitary-specific transcription factor which has responsible for pituitary development and hormone expression in mammals. The main function of <I>PIT1</I> is for binding and trans-activity promoters of both Growth Hormone (GH) and Prolactin (PRL) gene. It was subjected to different molecular studies as a key role for understanding genetic variation in dairy cattle. This study was aimed to detect the polymorphism in a part of <I>PIT1</I> gene, exon 6 in Najdi cattle. DNA was extracted from blood sample collected from 84 Najdi Cattle and submitted for Polymerase Chain Reaction (PCR) followed by digestion with <I>HinfI</I> restriction enzyme. Base on PCR-RFLP method, it found that the frequency of the A and B alleles of this gene for Najdi Cattle were 18.45 and 81.55%, respectively. The genotypic frequencies of AA, AB and BB were 3.57, 29.76 and 66.66%, respectively. &#967;<SUP>2</SUP>-test indicated that these two populations were at Hardy-Weinberg equilibrium. Thus, the selection base on these loci in the population is possible to be done.
ER  -