TY  - JOUR
T1  - Detection of Allelic Polymorphism in a Gene of the Major Histocompatibility Complex of Iranian Buffalo
AU - Rahimnahal, S. AU - Fayazi, J. AU - Mirzadeh, Kh. AU - Beigi Nassiry, M.T. AU - Roshanfekr, H. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 14
SP  - 1902
EP  - 1904
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.1902.1904
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.1902.1904
KW  - BoLA-DRB3
KW  -buffalo
KW  -PCR-RFLP
KW  -polymorphism
KW  -MGC
KW  -Iran
AB  - The Bovine Lymphocyte Antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to CD<SUP>4</SUP> T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. Since different alleles favor the binding of different peptides, DRB3 has been extensively evaluated as a candidate marker for associations with various bovine diseases and immunological traits. Therefore in this study, the genetic diversity of the bovine class II DRB3 locus in the buffalo population in Khouzestan Province of Iran investigated by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method (PCR-RFLP). Buffalo DNA isolated from whole blood. A hemi-nested PCR followed by digestion with restriction endonucleases Hae III conducted on the DNA.
ER  -