TY  - JOUR
T1  - Neutralization of Tetanus Neurotoxin with Various Botulinum Antitoxins 
 (A-F) in a Study to Isolate <I>Clostridium botulinum</I> from Field Samples
AU - Saeed, E.M.A. AU - Bohnel, H. AU - Gessler, F. 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 13
SP  - 1864
EP  - 1867
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.1864.1867
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.1864.1867
KW  - Neutralization
KW  -tetanus neurotoxin
KW  -botulinum antitoxins
KW  -mouse bioassay
KW  -antibodies
KW  -Germany
AB  - In a study to isolate <I>Clostridium botulinum</I> from field samples, 53 <I>C. botulinum</I>-like isolates were found neutralizable by botulinum antitoxins using the standard mouse bioassay. The neutralization results of 14 of them were found false-positive due to contamination with <I>C. tetani</I> which was separated from these isolates and found neutralizable by the same botulinum antitoxin types. Due to this result, <I>C. tetani</I> was isolated from other five field samples to test their toxins by botulinum antitoxins. They were also found neutralizable by certain botulinum antitoxins. Supernatants of <I>C. tetani</I> isolates were confirmed by being neutralized by tetanus antitoxin. Tetani toxins were found neutralizable by different botulinum antitoxins (A-F) but mainly by polyvalent type ABE (Aventis Behring, Marburg) and type CD mixture (Onderstepoort Veterinary Institute, Onderstepoort). These results were mainly attributed to the non-specificity of the proteins of some (at least ABE and CD) botulinum antitoxin hyperimmune sera which may contain antibodies against <I>C. tetani</I> as well.
ER  -