TY - JOUR T1 - Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes AU - Yamamoto, Makiko AU - Taniguchi, Yukio AU - Kano, Koichiro AU - Yamada, Takahisa JO - Journal of Animal and Veterinary Advances VL - 9 IS - 11 SP - 1639 EP - 1645 PY - 2010 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2010.1639.1645 UR - https://makhillpublications.co/view-article.php?doi=javaa.2010.1639.1645 KW - Adipose depotsite KW -C/EBP KW -differentiation KW -gene expression KW -leptin KW -PPAR³ KW -preadipocyte KW -proliferation AB - Adipose depotsite-specific variability in physiological characteristics of preadipocytes could be in part, intrinsic to the cells and become evident through in vitro proliferation and differentiation-dependent gene expression patterns in preadipocyte in the cell culture. This study was designed to characterize proliferation and differentiation-dependent gene expression patterns intrinsic to intramuscular, perirenal and subcutaneous preadipocytes. About 3 type preadipocytes are prepared using the ceiling culture method from bovine intramuscular, perirenal and are demonstrated subcutaneous adipose depots of a single Japanese Black steer. Adipose depot site-specific proliferation and differentiation-dependent gene expression patterns intrinsic to preadipocyte by comparing the mRNA levels of CCAAT enhancer binding protein C/EBPα, C/EBPβ, C/EBPδ, Peroxisome Proliferator-Activated Receptor γ (PPARγ) and leptin before and after the induction of differentiation among culture of the 3 type preadipocytes. These results may suggest the existence of the site-specific preadipocyte-intrinsic proliferation and differentiation program. ER -