TY  - JOUR
T1  - Effects of Extract of Green Tea and Ginseng on Pancreatic Beta Cells and Levels of Serum Glucose, Insulin, Cholesterol and Triglycerides in Rats with Experimentally Streptozotocin-Induced Diabetes: A Histochemical and Immunohistochemical Study
AU - H. Yoruk, Ibrahim AU - Yoruk, Mecit AU - Karaca, Turan AU - Uslu, Sema 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 1
SP  - 102
EP  - 107
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.102.107
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.102.107
KW  - hypoglycaemia
KW  -pancreatic b-cell
KW  -diabetes mellitus
KW  -ginseng
KW  -Green tea
KW  -streptozotocin
AB  - This study investigated the effects of oral administration of extract of green tea (<I>Camellia sinensis</I>) and ginseng (American ginseng<I>-Panax quinquefolium L</I>.), given alone or together, on pancreatic &#946;-cells, blood glucose, insulin, cholesterol and triglyceride levels in rats with experimental diabetes induced by a single injection of Streptozotocin (STZ) (60 mg kg<SUP>-1</SUP>, i.p.). Fifty adult Wistar Albino rats were used, 10 in each of these five treatment groups: Group A: healthy controls, Group B: STZ-induced diabetes (untreated), Group C: STZ-induced diabetes plus green tea extract (100 mg/kg/daily), Group D: STZ-induced diabetes plus ginseng root (400 mg/kg/daily) and Group E: STZ-induced diabetes plus ginseng root + green tea extracts as before. At the end of the 6 weeks experiment, blood samples were analysed for blood glucose, insulin, cholesterol and triglyceride levels and samples of pancreatic tissue were examined histochemically and immunohistochemically for endocrine islets and &#946;-cells. Overall, body weight decreased in groups B and C, serum insulin concentrations decreased slightly in groups C-E and total triglyceride levels of blood decreased significantly (p&lt;0.05) in groups B and C compared with control, D and E groups. Histopathological examination showed that degenerative changes in pancreatic &#946;-cells in STZ-treated rats were minimised to near normal morphology by administration of ginseng (Group D) and ginseng+green tea (Group E) and there was increased intensity of immunohistochemical staining for insulin in these groups. Degeneration of islets of Langerhans &#946;-cells and weak insulin staining was observed for green tea alone (Group C). These findings demonstrate that ginseng or combined ginseng + green tea decreases blood glucose levels in diabetic rats and increases preservation of &#946;-cells, perhaps by lowering oxidative stress.
ER  -