TY  - JOUR
T1  - Genotyping of Kappa-Casein Locus by PCR-RFLP in Brown Swiss Cattle Breed
AU - , Unsal Dogru AU - , Memis Ozdemir 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 4
SP  - 779
EP  - 781
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.779.781
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.779.781
KW  - Kappa-casein
KW  -PCR-RFLP
KW  -genetic marker
KW  -polymorphism
KW  -brown swiss
KW  -cattle
AB  - A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) test was performed on DNA samples extracted from blood samples of Brown Swiss cattle to detect genotype frequency of the bovine kappa-casein (CNS3) locus. A 351 bp fragment of CSN3 was amplified and digested with Hinf I restriction enzymes. Samples were loaded on agarose jel (3%) and genotyped under UV light. Three genotypes were observed, frequencies were 19.35, 20.43 and 60.22% for AA, BB and AB, respectively. Two genetic variants CSN3 A and CNS3 B were identified and the allelic frequencies were estimated as 0.495 and 0.505, respectively. It was concluded that the population was in Hardy-Weinberg equilibrum.
ER  -