TY  - JOUR
T1  - Isolation, Purification and Culture of Spermatogonia in Chicken
AU - Musa, H.H. AU - Chen, G.H. AU - Qin, J. AU - Sun, S.Y. AU - Zhou, G.Y. AU - Li, B.C. AU - Wu, H. AU - Wu, X.S. 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 12
SP  - 2418
EP  - 2423
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.2418.2423
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.2418.2423
KW  - culture
KW  -purification
KW  -isolation
KW  -spermatogonia
KW  -Chicken
AB  - To isolate, purify and culture spermotogonia from chicken testicular tissues, a procedure of enzymatic digestion and percoll density centrifugation was adopted for the single cell suspension to obtain purified spermatogonia. The results showed that, using the same purification method, the purity of spermatogonia gained from 6 days old chicken embryo was more than from 13, 15 and 19 days of age; adhesion purification step led to a harvest of 82% of total spermatogonia, which was 15.6% higher than that of direct isolation method; the adhesion time and survival time of spermotogonia before percoll density gradient centrifugation was earlier and longer than after precoll density gradient centrifugation.
ER  -