TY  - JOUR
T1  - Detection of Thermophilic <I>Campylobacter </I>sp. in Unpacked Broiler Carcasses in Retail Markets of Afyonkarahisar and Confirmation <I>C. jejuni</I> Isolates Using PCR
AU - Pamuk, Sebnem AU - Akgun, Sadi 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 10
SP  - 2063
EP  - 2068
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.2063.2068
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.2063.2068
KW  - Thermophilic Campylobacter
KW  -chicken carcasses
KW  -PCR
KW  -C. jejuni
KW  -Turkey
AB  - The aim of this present study is to detect thermophilic <I>Campylobacter</I> species in chicken carcasses sold unpackaged in Afyonkarahisar and to confirm <I>C. jejuni</I> isolates by using a PCR test based on the <I>ceuE</I> gene. A total of 210 chicken carcasses were collected from various markets from May till the end of October in 2004. Thermophilic <I>Campylobacter</I> sp. was isolated from 141 (67%) of 210 chicken carcass samples by classical culture method. The identification of 419 thermophilic <I>Campylobacter</I> sp. isolates obtained from chicken carcass samples were; 57.04% (239) <I>C. jejuni</I>, 40.57% (170) <I>C. coli</I> and 2.39% (10) <I>C. lari.</I> PCR method was applied to 101 of 239 <I>C. jejuni</I> isolates obtained from the chicken carcasses (n = 101). According to it 62 (61.38%) of 101 isolates were confirmed by PCR. About 28 (45.16%) of 62 isolates were confirmed to be <I>C. jejuni </I>were detected to be resistant to nalidixic acid.
ER  -