TY  - JOUR
T1  - Comparing <I>in vitro</I> Embryonic Development of Bovine Oocytes Cultured in G1.3/G2.3 Sequential Culture Media and CR1aa Medium
AU - , Mesut Cevik AU - , Hakan Sagirkaya AU - , Arzu Tas AU - , Tolga Akkoc AU - , Haydar Bagis AU - , Sezen Arat 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 6
SP  - 1185
EP  - 1189
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.1185.1189
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.1185.1189
KW  - Bovine
KW  -IVF
KW  -embryo culture
KW  -G1.3/G2.3 sequential media
KW  -CR1aa
AB  - <I>In vitro</I> embryo culture is an important step of <I>in vitro</I> production of bovine embryos. The aim of this study was to compare the <I>in vitro</I> development of bovine embryos in G1.3/G2.3 sequential culture media and CR1aa culture medium. Oocytes were obtained from ovaries of slaughtered cows and matured in TCM-199 medium in humidified air containing 5% CO<SUB>2</SUB> at 39&deg;C. Matured oocytes were fertilized <I>in vitro</I> using frozen bull sperm prepared with Percoll separation. After fertilization, the presumptive zygotes were denuded from cumulus cells and randomly allotted for 2 treatments: Culture in CR1aa (n = 116) and G1.3/G2.3 (n = 125). The embryo culture was carried out in 50 &#956;L droplets of the media that were placed in incubator containing 6% CO<SUB>2</SUB>, 5% O<SUB>2</SUB> and 89% N<SUB>2</SUB>. The embryos were evaluated on days 3, 5 and 7 following <I>in vitro</I> fertilization. The difference of cleavage rates between 2 treatments was not found significant (p&gt;0.05). However, significant difference was observed between 2 treatments at the level of the obtaining rate of morula and blastocyst (p&lt;0.05). The results showed that G1.3/G2.3 sequential media significantly favoured developing of morula and blastocysts in comparison to CR1aa medium (32.0% versus 10.40% versus 18.96 and 2.58%, respectively).
ER  -