TY  - JOUR
T1  - Sex Identification of Seven Species of Cranes in China by PCR
AU - , Bao Wen-Bin AU - , Wu Sheng-Long AU - , Zhang Hong-Xia AU - , Luan De-Qin AU - , H.H. Musa AU - , Chen Guo-Hong 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 6
SP  - 1137
EP  - 1140
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.1137.1140
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.1137.1140
KW  - Crane
KW  -EE0.6 sequence
KW  -sex identification
KW  -PCR
KW  -DNA
KW  -China
AB  - Sexes of cranes cannot be distinguished visually due to the morphological similarities between male and female. To facilitate the protection program for the endangered birds, red-crowned cranes in particular, genomic DNA was extracted from feather samples and a PCR-based sexing method was established using three primer combinations of avian sex-specific EE0.6 sequence. The specificity of the PCR was confirmed by amplifying genomic DNA from 22 known-sex cranes of seven species. After 30 cycles of amplification, easily distinguishable single bands for males and two bands for females were displayed by agarose gel electrophoresis. The sexes of 50 unknown-sex cranes of seven species were successfully identified using the PCR. The noninvasive PCR-based sexing method can not only facilitate the human-assisted breeding of cranes, but also provide useful reference for accurate sexing of other endangered birds.
ER  -