TY  - JOUR
T1  - Genetic Polymorphism FecB and BMP15 Genes and Its Association with Litter Size in Sangsari Sheep Breed of Iran
AU - , Mohammad Mehdi Kasiriyan AU - , Hasan Hafezeyan AU - , Hadi Sayahzadeh AU - , Reza Jamshidi AU - , Saied Reza Asghari AU - , Gholam Hossein Irajeyan AU - , Hamzeh Buesagh 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 5
SP  - 1025
EP  - 1031
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.1025.1031
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.1025.1031
KW  - PCR
KW  -polymorphism
KW  -BMP15
KW  -FecB
KW  -sangsari sheep
KW  -genetic polymorphism
AB  - Phenotypic evaluation and culling of candidate animals for traits by applying traditional animal breeding are usually costly tasks, which require considerable time to be carried out. Molecular genetic as an alternative method, enables animal breeders to select eligible animals for the desirable trait(s) at their earlier ages. Selection based upon markers could result in increasing accuracy as well as selection response of animals. This study was carried out to evaluate the genetic polymorphisms in BMP15 and FecB in Iranian Sangsari sheep breed. Blood samples were taken from 150 Sangsari sheep (140 ewes, 10 rams) from sheep in Damghan genetic modification center and the genomic DNA was extracted using salting out method. After the extraction and quantitative and qualitative tests (80% spectrophotometer and gel agarose 8%) the required amounts for each Polymerase Chain Reaction (PCR) were specified. Using 2 pairs of specific primers, 2 DNA fragments were amplified from exon 2 of BMP15 (141 bp) and FecB (190 bp) genes. The resulted PCR products were digested using HinfI and AvaΠ restriction enzymes for BMP15 and FecB genes, respectively. Genotypes of each individual were detected by agarose gel electrophoresis. Restriction digested of PCR products for BMP15 locus with Hinf I enzyme showed a C to T transition. BMP15 and FecB luci were not polymorphic. Further studied required to evaluate the relationship of different genotypes with litter size and ovulation rate.
ER  -