TY  - JOUR
T1  - <I>In situ</I> Analysis of CD4, CD8 and Mast Cells in Lung of <I>Mycoplasma hyopneumoniae</I> Experimentally Infected Pigs
AU - , Tonatiuh Alejandro Cruz Sanchez AU - , Socorro Sandra Martinez Robles AU - , German Garrido Farina AU - , Jorge Tortora Perez AU - , Susana Mendoza Elvira AU - , Abel Ciprian Carrasco AU - , Eliseo Hernandez Baumgharten AU - , Andres Romero Rojas AU - , Marco Antonio Vega-Lopez 
JO  - Journal of Animal and Veterinary Advances
VL  - 7
IS  - 6
SP  - 752
EP  - 758
PY  - 2008
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2008.752.758
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2008.752.758
KW  - Mycoplasma hyopneumoniae
KW  -Immune response
KW  -CD4
KW  -CD8
KW  -Mast cell
KW  -swine lung
AB  - Mast cells (MC) and CD4<SUP>+</SUP> and CD8<SUP>+</SUP> cell subpopulations, from the apical border of the right cranial lobe of lungs of pigs experimentally infected with <I>Mycoplasma hyopneumoniae</I>, were histologically (MC) and immunohistochemically (CD4 and CD8) analyzed during the first 20 days post infection (PI). Twelve weaned,  four-week-old  healthy  pigs  were endotracheally inoculated with a lung homogenate infected with <I>M. hyopneumoniae</I>, strain 194 and 6 control animals were inoculated with Friis medium only. A control animal and two infected animals were euthanized at days 0, 4, 8, 12, 16 and 20 PI. <I>M. hyopneumoniae</I> induced an early increase of CD4 cells at day 4 PI (p&lt;0.001) and at day 8 PI there was a significant and coincident increase in all three cell populations (p&lt;0.001), which intermittently persisted until day 20 (CD8 p&lt;0.001, MC p&lt;0.001). The results of this work suggest that infection with <I>M. hyopneumoniae</I> induces an early response of CD4+ cells, followed by an infiltration of CD8+ cells and MCs, possibly related to the pathophysiological events of the disease, since, despite the evident immune infiltration, the infection continued its course.
ER  -