TY  - JOUR
T1  - Microvesicles of <I>Actinobacillus seminis</I> Show Proteolytic Activity
AU - , Arturo Jimenez-Pacheco AU - , Alma Nunez-del Arco AU - , Enrique Salas-Tellez 
JO  - Journal of Animal and Veterinary Advances
VL  - 7
IS  - 5
SP  - 616
EP  - 619
PY  - 2008
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2008.616.619
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2008.616.619
KW  - Actinobacillus seminis
KW  -proteases
KW  -microvesicles
KW  -proleolytic activity
AB  - The objective of the present study was to identify proteolytic activity in microvesicles (MVs) from <I>Actinobacillus seminis</I>. A reference strain <I>A. seminis</I> ATCC 15768 and a field strain isolated were used. The microvesicles were obtained from ultracentrifugation from 48 h cultures. The proteolytic activity was determined in PAGE-SDS (12%) copolimerized with gelatin at 1%. The protein profile of the microvesicles was also established. The size of MVs was determined with transmission electron microscopy and it was determined to range  from  100-150 nm. The protein profile rendered a 75 kDa  protein, previously reported to be specific for <I>A. seminis</I>. Proteolytic activity was detected in the bands located at 79.5, 59, 40, 27 and 13.5 kDa. These results demonstrated the presence of proteolytic activity in <I>A. seminis</I> MVs. Further investigation on protease characterization and assessment of their importance in ovine epididimitis pathogenesis is needed.
ER  -