TY  - JOUR
T1  - Effect of the Addition of Follicular Fluid on <I>in vitro</I> Maturation Media for Bovine Oocytes
AU - , C.E. Larocca AU - , I. Lago AU - , M. Viqueira AU - , R. Correa AU - , W. Perez AU - , J. Caravia 
JO  - Journal of Animal and Veterinary Advances
VL  - 7
IS  - 1
SP  - 21
EP  - 23
PY  - 2008
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2008.21.23
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2008.21.23
KW  - Follicular fluid
KW  -maturation media
KW  -bovine oocytes
KW  -addition
AB  - The objective of the present research was to evaluate the effect of the addition of bovine Follicular Fluid (FF) to the <I>In vitro</I> Maturation media (IVM) of bovine oocytes. The Cumulus Oocyte Complexes (COC) were  obtained  by  aspiration  with  PBS+bovine fetal serum (CS)+Antibiotics (ATB) from follicles measuring 2-5 mm in diameter from ovaries collected from a slaughter house. The FF was extracted from 15 mm follicles and was inactivated. In total, 718 COC (A and B) were selected and were randomly divided into two groups: G1 (Control, 329 COC), TCM+5% CS+ATB; G2 (FF, 389 COC), TCM-199/10% FF+ATB. Each group was then subdivided into 4 subgroups: [G1T1&gt; 18h (69 COC); 20h (82 COC); G1T3: 22h (99 COC) and G1T4: 24 h (100 COC)]. COC were matured at 38.5°C, 5% CO<SUP>2</SUP> and 99% humidity. The IVM of oocytes was evaluated by observing the first polar body using a stereoscopic comb (40X) and by visualizing the metaphasic plate of oocytes stained with 1% acetic orceine under the microscope (400X). The total number of mature COC for each group was: [G1T1: 18h (17), G1T2: 20h (28), G1T3: 22h (31) and G1T4: 24h (48); G2T1: 18h (38), G2T2: 20h (46), G2T3: 22h (47) and G2T4: 24h (49)]. Chi-square test was used with a significance level of 5%. These results show that the addition of FF enhaces the maturation percentages at 18, 20 and 22 h, with the best results obtained at 22 h. At 24 h percentages are similar between the 2 groups.
ER  -